228-怎么区分不同的测序数据phred64 or phred33

刘小泽写于2021.1.22

示例

比如给你一段测序数据(这是chipseq的数据):

@SRR866627_nutlin_p53.1 HWI-ST571:161:D0YP4ACXX:3:1101:1437:2055 length=51
NCGAAAGACTGCTGGCCGACGTCGAGGTCCCGATTGTCGGCGTCGGCGGCA
+SRR866627_nutlin_p53.1 HWI-ST571:161:D0YP4ACXX:3:1101:1437:2055 length=51
#1:AABDDH<?CF<CFHH@D:??FAEE6?FHDAA=@CF@4@EBA88@;575
@SRR866627_nutlin_p53.2 HWI-ST571:161:D0YP4ACXX:3:1101:1423:2163 length=51
GAAATACTGTCTCTACTAAAAATACAAAAAGTAGCCAGGCGTCGTGGTGCA
+SRR866627_nutlin_p53.2 HWI-ST571:161:D0YP4ACXX:3:1101:1423:2163 length=51
B@CFFFFFHHHHGIJJJJIJJJIJJJJIJJIEGHIJJJJIJIIIHIE=FGC

答案是:phred 33数据

Tips

因为上面的第3行,有数字,所以就是phred33

Yunze Liu
Yunze Liu
Bioinformatics Sharer

Co-founder of Bioinfoplanet(生信星球)

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